PHYSICOCHEMICAL AND ORGANOLEPTIC EVALUATION OF ARABICA COFFEE SUBJECTED TO CONTROLLED FERMENTATION
DOI:
https://doi.org/10.31413/nat.v13i4.20393Keywords:
coffe, fermentation, ABTS, DPPH, FRAP, PolyphenolsAbstract
The objective of this study was to identify the independent effect of the addition of S. cerevisiae S33 and S04 strains and L. casei on the physicochemical attributes of green coffee. For both the quantification of total polyphenols and antioxidants, results were expressed as milligrams of gallic acid equivalent per milliliter of extract. For DPPH determination, FRAP was used as the Trolox standard.
According to the Tukey test (P < 0.05), there was no statistically significant difference in the present study for the determination of soluble solids (or Brix), pH, total polyphenols, antioxidants by ABTS, or antioxidants by FRAP. On the other hand, statistical differences were found in antioxidants using DPPH, as well as for organoleptic attributes, which show that the S. cerevisiae S-33 treatment with L. casei was characterized by greater sweetness, body, and acidity, presenting a high positive linear tension, presenting a direct association with these attributes. This may be due to the fact that coffee phenols are hydrophilic, which is why differences were observed for DPPH, which implies a determining aspect in the processes of determining antioxidants. and better sensory attributes through the addition of microorganisms.
References
AOAC_ASSOCIATION OF OFFICIAL ANALYTICAL CHEMISTS. Official Methods of Analysis. Washington, DC: AOAC, 1984. Available at: https://www.aoac.org/official-methods-of-analysis/ B
BARBA, C.; TINOCO, F. Influencia de la aplicación de dos tipos de levaduras y el tiempo de fermentación, en la calidad organoléptica de taza de Coffea arabica var. típica de la finca Agroloja. 70f. Universidad Nacional de Loja, Loja, Ecuador, 2023. https://dspace.unl.edu.ec/jspui/handle/123456789/27154
BERTRAND, B.; BOULANGER, R.; DUSSERT, S.; RIBEYRE, F.; BERTHIOT, L.; DESCROIX, F.; JOËT, T. Climatic factors directly impact the volatile organic compound fingerprint in green Arabica coffee bean as well as coffee beverage quality. Food Chemistry, v. 135, n. 4, p. 2575-2583, 2012. https://doi.org/10.1016/j.foodchem.2012.06.060
BRESSANI, A. P. P.; BATISTA, N. N.; FERREIRA, G.; MARTINEZ, S. J.; SIMÃO, J. B. P.; DIAS, D. R.; SCHWAN, R. F. Characterization of bioactive, chemical, and sensory compounds from fermented coffees with different yeast species. Food Research International, v. 150, e110755, 2021. https://doi.org/10.1016/j.foodres.2021.110755
CEVALLOS, K.; SERRANO, J. Aprovechamiento de los compuestos activos a partir de extractos de residuos agroindustriales como sustituto de los conservantes sintéticos convencionales de alimentos. 111f. Dissertación [Químico de Alimentos] - Universidad Central del Ecuador, Quito, Ecuador. 2023. Available on: http://www.dspace.uce.edu.ec/handle/25000/31647.
ELHALISA, H.; COXA, J.; FRANKB, D., ZHAOA, J. The crucial role of yeasts in the wet fermentation of coffee beans and quality, Australia. International Journal of Food Microbiology, v. 333, e108796, 2020. https://doi.org/10.1016/j.ijfoodmicro.2020.108796
ELHALISA, H.; COXA, J.; FRANKB, D.; ZHAOA, J. Microbiological and chemical characteristics of wet coffee fermentation inoculated with Hansinaspora uvarum and Pichia kudriavzevii and their impact on coffee sensory quality, Australia. Frontiers in Microbiology, v. 12, e108796, 2021. https://doi.org/10.3389/fmicb.2021.713969
FESSARD, A.; KAPOOR, A.; PATCHE, J.; ASSEMAT, S.; HOARAU, M.; BOURDON, E.; BAHORUN, T.; REMIZE, F. Lactic fermentation as an efficient tool to enhance the antioxidant activity of tropical fruit juices and teas, France. Microorganisms, v. 5, n. 20, e23, 2017. https://doi.org/10.3390/microorganisms5020023
FUJIOKA, K.; SHIBAMOTO, T. Chlorogenic acid and caffeine contents in various commercial brewed coffee, California. Food Chemistry, v. 106, n. 1, p. 217-221, 2008. https://doi.org/10.1016/j.foodchem.2007.05.091
GUZMÁN, S.; MOTATO, K. Potencial tecnológico de cepas nativas de levaduras aisladas en procesos de fermentación natural de café para la producción de cafés especiales. Revista Colombiana de Biotecnología, v. 18 n. 1, p. 20-31, 2021. https://doi.org/10.15446/rev.colomb.biote.v26n1.113115
HUACCHA-HERRERA, C. Efecto del grado de tostado en el contenido de polifenoles totales, actividad antioxidante y calidad en taza del café, variedad typica y Bourbón. 116f. Tesis de Grado [Ingeniero en Industrias Alimentarias] - Universidad Nacional Agraria de la Selva, Tingo Maria, Perú, 2016. Available on: https://hdl.handle.net/20.500.14292/1258
IANNIELLO, R.; RICCIARDI, A.; PARENTE, E.; TRAMUTOLA, A.; REALE, A.; ZOTTA, T. Aeration and supplementation with heme and menaquinone affect survival to stresses and antioxidant capability of Lactobacillus casei strains. LWT - Food Science and Technology, v. 60, n. 2, p. 817-824, 2015. https://doi.org/10.1016/j.lwt.2014.10.020
LALLEMAND. Levadura natural seleccionada LALCAFÉTM. 2024. Available on: https://www.lalcafebylallemand.com/es/productos/lalcafe-inicio/. Accessed at: 05 Dec. 2025.
LÓPEZ, P.; IRACHETA, L.; CASTELLANOS, J.; MÉNDEZ, I.; AGUIRRE, J.; GUTIÉRREZ, A.; OJEDA, M.; PÉREZ, B. Variación en la tolerancia a desinfectantes de genotipos élite de Coffea spp. cultivados in vitro. Revista Méxicana de Ciencias Agrícolas, v. 2, n. 5, p. 645-657, oct. 2011. http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S2007-09342011000500002
LÓPEZ, T.; PRADO, A.; NEVÁREZ, G.; CONTRERAS, J.; RODRÍGUEZ, R.; AGUILAR, C. Incremento de la capacidad antioxidante de extractos de pulpa de café por fermentación láctica en medio sólido. CYTA - Journal of Food, v. 11, n. 4, p. 359-365, 2013. http://dx.doi.org/10.1080/19476337.2013.773563
OKTAVIANI, L.; ASTUTI, I.; ROSMIATI, M.; ABDUH, M. Y. Fermentation of coffee pulp using indigenous lactic acid bacteria with simultaneous aeration to produce cascara with a high antioxidant activity. Heliyon, v. 6, n. 7, e04462, 2020. https://doi.org/10.1016/j.heliyon.2020.e04462
OZGEN, M.; REESE, R.; TULIO, A.; SCHEERENS, J.; MILLER, A. Modified 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic Acid (ABTS) Method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2′-Diphenyl-1-picrylhydrazyl (DPPH) methods. Journal of Agricultural and Food Chemistry, v. 54, n. 4, p. 1151-1157, 2006. https://doi.org/10.1021/jf051960d
PALACIOS, F.; HUAYAMA, P. Análisis sensorial del café catimor (Coffea arabica) inoculado con cepas de Saccharomyces cerevisiae en dos sistemas de fermentación. Revista Pakamuros, v. 11 n. 1, p. 123-130, 2023. https://doi.org/10.37787/wm10gf28
PAZMIÑO, J.; RUÍZ, A. Evaluación sensorial y de compuestos volátiles aromáticos del café (Coffea arábica var. Caturra Chiroso) de tres orígenes geográficos de Antioquia. Ciencia y Tecnología Agropecuaria, v. 24, n. 1, e2846, 2023. https://doi.org/10.21930/rcta.vol24_num1_art:2846
PIVARAL, R.; CRUZ, J. Evaluación del efecto de Saccharomyces cerevisiae sobre la caracterización sensorial del café en dos sistemas de fermentación. 34f. Projeto especial de Graduação [Licenciatura em Agroindústria Alimentária] - Escuela Agrícola Panamericana, Zamorano, Honduras, 2018. Available on: https://bdigital.zamorano.edu/bitstream/11036/6232/1/AGI-2018-T019.pdf
RIBEIRO, L. S.; MIGUEL, M. G. C. P.; EVANGELISTA, S. R.; MARTINS, P. M. M.; VAN MULLEM, J.; BELIZARIO, M. H.; SCHWAN, R. F. Behavior of yeast inoculated during semi-dry coffee fermentation and the effect on chemical and sensorial properties of the final beverage. Food Research International, v. 92, p. 26-32, 2017. https://doi.org/10.1016/j.foodres.2016.12.011
SILVA, C. F.; VILELA, D. M.; DE SOUZA CORDEIRO, C.; DUARTE, W. F.; DIAS, D. R.; SCHWAN, R. F. Evaluation of a potential starter culture for enhance quality of coffee fermentation. World Journal of Microbiology and Biotechnology, v. 29, n. 2, p. 235-247, 2013. https://doi.org/10.1007/s11274-012-1175-2
SCA_Specialty Coffee Association. Sample Preparation and Tasting Mechanics. 102 – 2024. Available on: https://sca.coffee/research/coffee-standards
TAMILMANI, P.; PANDEY, M. C. Optimization and Evaluation of phenolic compounds and their antioxidant activity from coffee beans. International Journal of Advanced Research, v. 3, n. 4, p. 296-306, 2015.
TAPIERO, J.; MÉNDEZ, R. Modelo matemático del proceso de fermentación del café. 75f. Trabajo de Investigación [Licenciatura en Matemáticas] - Universidad del Quindío, Colombia, 2023. Available on: https://bdigital.uniquindio.edu.co/server/api/core/bitstreams/c7108e7e-dbc3-409d-9566-bce51ad22b33/content
THAIPONG, K.; BOONPRAKOB, U.; CROSBY, K.; CISNEROS-ZEVALLOS, L.; HAWKINS BYRNE, D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis, v. 19, n. 6-7, p. 669-675, 2006. https://doi.org/10.1016/j.jfca.2006.01.003
URRUNAGA DE ROZAS, E. J.; TORRES POLANCO, J. G.; ALOSILLA ROBLES, C. S. A.; PUMA CAPARO, B. P. P. Evaluación de las características fisicoquímicas, cromatográficas y actividad antioxidante del café verde caracolillo y natural (Coffea arabica L.) de Umapata-Cusco. Q’EUÑA, v. 15, n. 2, p. 16-25, 2024. https://doi.org/10.51343/rq.v15i2.1570
WANG, C.; SUN, J.; LASSABLIERE, B.; YU, B.; LIU, S. Q. Coffee flavour modification through controlled fermentations of green coffee beans by Saccharomyces cerevisiae and Pichia kluyveri: Part I. Effects from individual yeasts. Food Research International, v. 136, e109588, 2020a. https://doi.org/10.1016/j.foodres.2020.109588
WANG, C.; SUN, J.; LASSABLIERE, B.; YU, B.; LIU, Q. Coffee flavour modification through controlled fermentation of green coffee beans by Saccharomyces cerevisiae and Pichia kluyveri: Part II. Mixed cultures with or without lactic acid bacteria. Food Research International, v. 136, e109452, 2020b. https://doi.org/10.1016/j.foodres.2020.109452
WANG, C.; SUN, J.; LASSABLIERE, B.; YU, B.; ZHAO, F.; ZHAO, F.; CHEN, Y.; LIU, S. Q. Potential of lactic acid bacteria to modulate coffee volatiles and effect of glucose supplementation: fermentation of green coffee beans and impact of coffee roasting. Journal of the Science of Food and Agriculture, v. 99 n.1, p. 409-420, 2018. https://doi.org/10.1002/jsfa.9202
Downloads
Published
Issue
Section
How to Cite
License
Copyright (c) 2025 Nativa

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright for articles published in this journal are the authors, with first publication rights granted to the journal. The journal shows open access, and articles are free to use, with proper attribution, in educational and non-commercial.
The articles published in this journal may be reproduced in part or used as a reference by other authors, provided that the source is quoted.

